Journal: American Journal of Translational Research

Article Title: EGFR/EGFRvIII partly regulates the tumourigenesis of glioblastoma through the SOX9-GLUT3 axis

doi:

Figure Lengend Snippet: The primers for RT-PCR

Article Snippet: Immunohistochemistry (IHC) Tissue sections from paraffin-embedded de-identified human glioblastoma specimens were dyed with antibodies against GLUT3 (1:60, Abcam), EGFR (1:100, Abcam), Ki67 (1:60, Abcam), and SOX9 (1:100, Abcam), and nonspecific IgG was applied as a negative control.

Techniques:

Journal: American Journal of Translational Research

Article Title: EGFR/EGFRvIII partly regulates the tumourigenesis of glioblastoma through the SOX9-GLUT3 axis

doi:

Figure Lengend Snippet: EGFR and GLUT3 were co-expressed in glioblastoma and were correlated with worse overall survival. (A) Elevated expression of EGFR and GLUT3 was related to worse overall survival in glioblastoma according to Rembrandt database analysis. (B) EGFR and GLUT3 were positively co-expressed at the mRNA level according to Rembrandt database analysis. (C) High levels of EGFR and GLUT3 were associated with poorer overall survival, and (D) EGFR and GLUT3 were co-expressed at the mRNA level via RT-PCR detection in glioblastoma in our collected samples. (E) IHC results showed that in EGFR-positive tissues (n=3), the expression of GLUT3 was relatively higher than that in EGFR-negative tissues (n=3). Original magnification: 40×; scale bar: 100 µm.

Article Snippet: Immunohistochemistry (IHC) Tissue sections from paraffin-embedded de-identified human glioblastoma specimens were dyed with antibodies against GLUT3 (1:60, Abcam), EGFR (1:100, Abcam), Ki67 (1:60, Abcam), and SOX9 (1:100, Abcam), and nonspecific IgG was applied as a negative control.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: American Journal of Translational Research

Article Title: EGFR/EGFRvIII partly regulates the tumourigenesis of glioblastoma through the SOX9-GLUT3 axis

doi:

Figure Lengend Snippet: EGFR/EGFRvIII upregulated GLUT3 expression in glioblastoma. (A and B) EGFR and GLUT3 were substantially overexpressed in U87-EGFRvIII and LN229-EGFRvIII cells at the mRNA (A) and protein (B) levels compared with those in wild-type U87 and LN229 cells. (C) Through treatment of U87-EGFRvIII cells with osimertinib and NSC228115, we found that EGFR promoted the expression of GLUT3. Mean ± SD. ***P<0.001. P values were calculated using two-tailed Student’s t-tests and one-way ANOVA.

Article Snippet: Immunohistochemistry (IHC) Tissue sections from paraffin-embedded de-identified human glioblastoma specimens were dyed with antibodies against GLUT3 (1:60, Abcam), EGFR (1:100, Abcam), Ki67 (1:60, Abcam), and SOX9 (1:100, Abcam), and nonspecific IgG was applied as a negative control.

Techniques: Expressing, Two Tailed Test

Journal: American Journal of Translational Research

Article Title: EGFR/EGFRvIII partly regulates the tumourigenesis of glioblastoma through the SOX9-GLUT3 axis

doi:

Figure Lengend Snippet: SOX9 was positively co-expressed with EGFR and GLUT3, and was correlated with worse overall survival. (A and B) Elevated expression of SOX9 was correlated with worse overall survival in glioblastoma according to Rembrandt database analysis (A) and our collected samples (B). (C and D) RT-PCR results showed that SOX9 was positively co-expressed with EGFR and GLUT3 at mRNA level. (E) Compared with SOX9-negative tissues (n=3), the expression of EGFR and GLUT3 was higher than that in SOX9-positive tissues (n=3). Original magnification: 40×; scale bar: 100 µm. (F) When EGFR/GLUT3 or GLUT3/SOX9 were simultaneously highly expressed, the prognosis for patients with glioblastoma was worsened.

Article Snippet: Immunohistochemistry (IHC) Tissue sections from paraffin-embedded de-identified human glioblastoma specimens were dyed with antibodies against GLUT3 (1:60, Abcam), EGFR (1:100, Abcam), Ki67 (1:60, Abcam), and SOX9 (1:100, Abcam), and nonspecific IgG was applied as a negative control.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: American Journal of Translational Research

Article Title: EGFR/EGFRvIII partly regulates the tumourigenesis of glioblastoma through the SOX9-GLUT3 axis

doi:

Figure Lengend Snippet: EGFR/EGFRvIII promoted the expression of GLUT3 through the combination of SOX9 and the GLUT3 promoter. (A) EGFR/EGFRvIII promoted the expression of SOX9, as detected by CHIP (D) assay. (B) There were binding sites between SOX9 and the promoter area of GLUT3 through bioinformatics analysis. (C and D) Inhibition of SOX9 reduced the expression of GLUT3 (C), while up-regulation of SOX9 promoted the expression of GLUT3 (D), as detected by CHIP assays. (E and F) Silencing SOX9 reduced the expression of GLUT3 (E), while overexpression of SOX9 promoted the expression of GLUT3, as detected by luciferase reporter assays. (G) The potential workflow of EGFR/EGFRvIII in glioblastoma was listed. Mean ± SD. ***P<0.001. P values were calculated using one-way ANOVA. EGFRhigh represents EGFR/EGFRvIII high-expression and EGFRlow represents EGFR/EGFRvIII low-expression.

Article Snippet: Immunohistochemistry (IHC) Tissue sections from paraffin-embedded de-identified human glioblastoma specimens were dyed with antibodies against GLUT3 (1:60, Abcam), EGFR (1:100, Abcam), Ki67 (1:60, Abcam), and SOX9 (1:100, Abcam), and nonspecific IgG was applied as a negative control.

Techniques: Expressing, Binding Assay, Inhibition, Over Expression, Luciferase

Journal: Aging (Albany NY)

Article Title: High-fat diet induced discrepant peripheral and central nervous systems insulin resistance in APPswe/PS1dE9 and wild-type C57BL/6J mice

doi: 10.18632/aging.202262

Figure Lengend Snippet: Glucose transporters (GLUTs) protein expressions in liver, skeletal muscle and omental adipocyte ( n = 6 at least for each group). ( A ) GLUT2 protein expression in liver tissue; ( B ) GLUT4 protein expression in muscle; ( C ) GLUT4 protein expression in omental adipocytes. a: control diet-treated C57 mice; b: 60% HFD-treated C57 mice; c: control diet-treated APP/PS1 mice; d: 60% HFD-treated APP/PS1mice.Scalebar:20μm.

Article Snippet: IHC was performed on paraffin-embedded sections using antibodies directed against GLUT2, (1:100 dilution, cell signaling technology, USA), GLUT3, (1:100 dilution, cell signaling technology, USA), and GLUT4 (1:100 dilution, proteintech, USA).

Techniques: Expressing, Control